Pairwise full-sib crosses were performed according to a multifactorial design. Furthermore, we show that deletion of spo11a gene required for proper recombination in most studied organisms, resulted in very different effects in males and females where males were sterile while females produced deformed progeny.
Mosaicism was estimated only for cases, investigated by both methods CVS in total.
We used a custom-designed oligonucleotide microarray containingprobes covering the assembled zebrafish genome Zv7. Sexual Development3— Introduction to zebrafish sexual phenotypes Adult zebrafish males and females can be distinguished based on several sexually dimorphic phenotypes.
Additional five CNVRs showed apparent family-specific sex linkage Table 2 and were further analyzed by PCR-based methods with additional two offspring individuals one male and one female from the same families. All these features point toward a species without a predominant chromosomal sex determination system .
Zebrafish also shared many of the common candidate genes involved in gonad differentiation refer to Table 2 of [ 21 ]. The Evolution of Sex Determination.
Altogether, offspring from 26 fourth generation families were grown to maturity and sexed Fig. The phenotype connected to POU1F1 mutations is characterized by profound GH and PRL deficiencies, variable degrees of TSH deficiency, severe proportional short stature, atypical facies and feeding difficulties in infancy.
The result of the QF-PCR analysis for rapid aneuploidy screening was normal, but add 8p was determined by conventional karyotyping.